New genus-specific primers for PCR identification of Rubrobacter strains

Genus- and species-specific PCR primers for the detection and identification of bifidobacteria.

16SrDNA-targeted genus- and species-specific PCR primers have been developed and used for the identification and detection of bifidobacteria. These primers cover all of the described species that inhabit the human gut, or occur in dairy products. Identification of cultured bifidobacteria using PCR primer pairs is rapid and accurate, being based on nucleic acid sequences. Detection of bifidobact...

Identification of Bifidobacterium Strains Isolated from Fecal Samples of Some Iranian Subjects Using 16SrRNA Gene Sequence Analysis and PCR-based Gene Specific Primers

For the first time in Iran 40 strains of Bifidobacterium were isolated from feces of Iranian subjects. By using phenotypic tests, 18 isolates were identified as Bifidobacterium longum, 10 as Bifidobacterium bifidum and one as Bifidobacterium catenolatum. In order to validate these results and also to identify other isolates that had not been identified by phenotypic tests, two methods of PCR wi...

Application of specific primers and polymerase chain reaction for identification of ralstonia solanacearum biovar2

a sensitive and specific pcr based method for identification of cryptosporidium sp. using new primers from 18s ribosomal rna

background: the main goal of the present study was to develop a new sensitive and specific pcr based method for identification of cryptosporidium sp. using novel primers from 18s ribosomal rna. cryptosporidi­osis in high-risk host groups particularly in neonates and immuno-compromised individuals may result in death. to the best of our knowledge this is the first study regarding develop a new p...

Serotype Specific Primers and Gel-Based RT-PCR Assays for ‘Typing’ African Horse Sickness Virus: Identification of Strains from Africa

African horse sickness is a devastating, transboundary animal disease, that is 'listed' by the Office International des Epizooties (OIE). Although attenuated, inactivated and subunit vaccines have been developed for African horse sickness virus (AHSV), these are serotype-specific and their effective deployment therefore relies on rapid and reliable identification of virus type. AHSV serotype is...